摘要
HMGB1（High mobility group box1 protein 1）作为DAMPs（Damageassociated molecular pattern, DAMPs），介导细胞间损伤与炎症信号的传递，在无菌炎症、自身免疫性疾病及癌症等病理过程中发挥关键作用，并作为药物靶点与生物标志物具有广泛研究价值。环维黄杨星D (cyclovirobuxine D, CVB-D)广泛应用于预防和治疗各种心血管疾病，具有显著的抗炎潜力。但目前暂时没有CVB-D与HMGB1相互作用的研究。故本研究在细胞水平和分子水平探究CVB-D与HMGB1相互作用及其分子机制。结果表明CVB-D抑制HMGB1诱导的炎症反应，这可能CVB-D能与HMGB1结合且能改变其构象有关。
关键词： HMGB1；CVB-D；小分子-蛋白相互作用
Abstract
As DAMPs, HMGB1 mediates the transmission of intercellular damage and inflammatory signals, plays a key role in pathological processes such as aseptic inflammation, autoimmune diseases and cancer, and has extensive research value as a drug target and biomarker. CVB-D is widely used in the prevention and treatment of various cardiovascular diseases, and has significant anti-inflammatory potential. However, there is currently no study on the interaction between CVB-D and HMGB1. Therefore, this study explored the interaction between CVB-D and HMGB1 and its molecular mechanism at the cellular and molecular levels. The results showed that CVB-D inhibited HMGB1-induced inflammatory response, which may be related to the fact that CVB-D can bind to HMGB1 and change its conformation.
Key words： HMGB1; CVB-D; Small molecule-protein interaction
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