Analysis of cytokine levels and circulating EPC in serum and placental tissue of patients with pregnancy-induced hypertension syndrome
妊娠高血压综合征患者血清、胎盘组织中细胞因子水平及循环EPC数量分析

摘要

目的 探讨妊娠高血压综合征作为远期高血压发生危险因素的可能性。方法 自制调研问卷，针对妇产科就诊患者进行调查，妊娠高血压综合征60例，列入观察组，非妊娠高血压综合征60例，列入对照组。检测各研究对象血清细胞因子的表达水平、胎盘组织中高度相关性细胞因子蛋白的表达水平以及外周血循环内皮细胞内皮祖细胞数量。结果 观察组孕妇血清VEGF水平低于对照组，血清AGEs、TGF-β1水平则高于对照组（均P＜0.05）。观察组孕妇胎盘组织中VEGF阳性表达率均低于对照组，而AGEs、TGF-β1阳性表达率均高于对照组（均P＜0.05）。观察组孕妇循环EPC数量低于对照组（P＜0.05）。血清及胎盘组织中VEGF水平与循环EPC数量与妊娠期高血压疾病严重程度呈负相关，AGEs、TGF-β1则与疾病严重程度呈正相关（均P＜0.05）。结论 妊高征孕妇血清和胎盘组织 VEGF含量均明显低于正常孕妇，且与其病情的严重性成负相关；而妊高征妇女血清和胎盘中的GF-β1含量明显高于正常孕妇，且与其病情的严重性有明显的正相关性；妊娠高血压综合征孕妇外周血循环内皮细胞内皮祖细胞数量显著低于正常妊娠孕妇，与病情严重程度呈负相关。四者与妊娠高血压疾病密切相关，在临床评估疾病进展中有重要意义。

Abstract

Objective: To explore the possibility of pregnancy-induced hypertension syndrome as a risk factor for long-term hypertension.
Methods: A self-made questionnaire was used to investigate the patients in obstetrics and gynecology. 60 cases of pregnancy-induced hypertension were included in the observation group, and 60 cases of non-pregnancy-induced hypertension were included in the control group. The expression levels of serum cytokines, the expression levels of highly correlated cytokine proteins in placental tissue and the number of endothelial progenitor cells in peripheral blood circulating endothelial cells were detected.
Results: The serum VEGF level of pregnant women in the observation group was lower than that in the control group, while the serum AGEs and TGF-β1 levels were higher than those in the control group (all P<0.05). The positive expression rates of VEGF in placenta tissue of pregnant women in the observation group were lower than those in the control group, while the positive expression rates of AGEs and TGF-β1 were higher than those in the control group (all P<0.05). The number of circulating EPCs in the observation group was lower than that in the control group (P<0.05). The levels of VEGF in serum and placental tissue and the number of circulating EPCs were negatively correlated with the severity of gestational hypertension, while AGEs and TGF-β1 were positively correlated with the severity of the disease (all P＜0.05).
Conclusion  : VEGF levels in serum and placenta of pregnant women with PIH were significantly lower than those in normal pregnant women, and were negatively correlated with the severity of their disease; while the levels of GF-β1 in serum and placenta of women with PIH were significantly higher than those in normal pregnant women, and There is a significant positive correlation with the severity of the disease; the number of endothelial progenitor cells in peripheral blood circulating endothelial cells of pregnant women with pregnancy-induced hypertension is significantly lower than that of normal pregnant women, which is negatively related to the severity of the disease. The four are closely related to gestational hypertension and are of great significance in clinical assessment of disease progression.